Transform & Plasmids Quiz

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Plasmid basics and transformation

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ILARIA SORRENTINO

Spain

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Transform & Plasmids Quiz


Transform & Plasmids QuizVersion en ligne Plasmid basics and transformation par ILARIA SORRENTINO 1 What is transformation in molecular biology? a Copying DNA during replication b RNA transcription from DNA c Protein synthesis of plasmids d Uptake of naked DNA by competent cells 2 Which plasmid feature allows replication in bacteria? a Ribosome binding site b Promoter c Origin of replication (ori) d Multiple cloning site (MCS) 3 Why do labs include an antibiotic resistance gene on plasmids? a To label the DNA backbone b To inactivate the plasmid c To select transformed cells on antibiotic plates d To increase plasmid size 4 Which technique commonly uses heat shock to introduce DNA into bacteria? a Lipofection b Chemical transformation with CaCl2 and heat shock c Microinjection d Electroporation 5 What best defines a plasmid? a A carbohydrate molecule b A protein complex c A circular DNA molecule that replicates independently in bacteria d A linear chromosome fragment 6 What is the purpose of a Multiple Cloning Site (MCS) on a plasmid? a Serve as a promoter sequence b Provide many restriction sites for inserting foreign DNA c Act as an origin of replication d Encode antibiotic resistance 7 What is the role of competent cells in transformation? a To degrade DNA b To increase DNA uptake during transformation c To fluoresce under UV light d To synthesize plasmids 8 How can transformed cells be identified effectively? a Only by PCR confirmation b No growth after transformation c Growth on plain media d Growth on antibiotic-containing selective media 9 What does the origin of replication (ori) determine? a Where and when the plasmid can replicate in the host b Whether the plasmid is linear or circular c The color of the colonies d Which promoter to use

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Transform & Plasmids Quiz

Test

À propos de cette activité

Créé par

Téléchargez la version pour jouer sur papier

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Transform & Plasmids QuizVersion en ligne

par ILARIA SORRENTINO

What is transformation in molecular biology?

Which plasmid feature allows replication in bacteria?

Why do labs include an antibiotic resistance gene on plasmids?

Which technique commonly uses heat shock to introduce DNA into bacteria?

What best defines a plasmid?

What is the purpose of a Multiple Cloning Site (MCS) on a plasmid?

What is the role of competent cells in transformation?

How can transformed cells be identified effectively?

What does the origin of replication (ori) determine?