TAQ Quest: PCR Basics

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PCR enzyme & TAQ knowledge

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Inobat Ravshanbekova

Uzbekistan

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TAQ Quest: PCR Basics


Froggy Jumps TAQ Quest: PCR BasicsVersion en ligne PCR enzyme & TAQ knowledge par Inobat Ravshanbekova 1 What organism provides the TAQ polymerase used in PCR? a Thermus aquaticus b Escherichia coli c Saccharomyces cerevisiae 2 TAQ polymerase is chosen for PCR mainly because it is a heat-tolerant b cold-sensitive c inhibited by salts 3 What is the main role of TAQ polymerase in PCR? a bind primers b synthesize DNA strands c cleave DNA 4 PCR step where DNA is denatured is a annealing b denaturation c extension 5 During PCR, primers bind to DNA during which step? a Extension b Annealing c Denaturation 6 Which temperature range is typical for TAQ polymerase activity? a 72°C around elongation b 95°C c 37°C 7 TAQ polymerase adds nucleotides in a Random order b 3' to 5' direction c 5' to 3' direction 8 Why is TAQ polymerase preferred in routine PCR? a It works at room temp b It remains active after heating cycles c It requires no primers 9 Which component pairs with primers to initiate synthesis? a RNA polymerase b DNA template c Ligase 10 What is the major limitation of TAQ polymerase? a Low processivity b No proof-reading activity c Cannot extend from primers

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TAQ Quest: PCR Basics

Froggy Jumps

À propos de cette activité

Créé par

Téléchargez la version pour jouer sur papier

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TAQ Quest: PCR BasicsVersion en ligne

par Inobat Ravshanbekova

What organism provides the TAQ polymerase used in PCR?

TAQ polymerase is chosen for PCR mainly because it is

What is the main role of TAQ polymerase in PCR?

PCR step where DNA is denatured is

During PCR, primers bind to DNA during which step?

Which temperature range is typical for TAQ polymerase activity?

TAQ polymerase adds nucleotides in

Why is TAQ polymerase preferred in routine PCR?

Which component pairs with primers to initiate synthesis?

What is the major limitation of TAQ polymerase?