Downstream Processing: DP in Biotech

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DP in biotech post-fermentation

Créé par

Nur Assyakirin binti Mohamed Paid

Malaysia

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Downstream Processing: DP in Biotech


Froggy Jumps Downstream Processing: DP in BiotechVersion en ligne DP in biotech post-fermentation par Nur Assyakirin binti Mohamed Paid 1 What is the primary function of solid-liquid separation? a Add nutrients b Enhance growth rate c Remove solids to obtain a clear solution 2 Which technique is used to purify proteins based on binding properties? a Fermentation b Lyophilization c Chromatography 3 Precipitation often recovers product by changing what? a Solubility b pH with no change in solubility c Temperature only 4 What drying technique is common for heat-sensitive materials? a Spray drying b Lyophilization c Condensation drying 5 Why are downstream purification steps essential? a Increase fermentation time b Reduce production cost only c Remove impurities to meet product specs 6 What is the stage that involves the cultivation and growth of microorganisms to produce desired products? a Upstream processing b Fermentation c Downstream processing 7 Which unit operations are commonly employed for solid-liquid separation in fermentation processes? a Chromatography and ultrafiltration b Centrifugation, filtration, and precipitation c Ultrafiltration and evaporation 8 What is the purpose of disruption of cells in downstream processing? a Improve product stability b Release intracellular products c Concentrate purified products 9 What is the primary principle behind bead milling for cell disruption? a Use of high-frequency sound waves b Sudden change in pressure drop c Agitation through bead-to-bead interaction 10 Which of the following is a method used for disruption of cells in downstream processing? a Chemical permeabilization b Centrifugation c Ultrafiltration 11 What is the common mechanism employed in homogenization to disrupt cells? a Sudden change of pressure drop b Use of high-frequency sound waves c Agitation with glass beads 12 What is the primary mechanism of separation in Gel Filtration (size exclusion) chromatography? a Separation based on charges b Separation based on size c Separation based on binding affinities 13 How does Ion-exchange chromatography separate molecules within a sample? a Based on size b Based on binding affinities c Based on charges 14 Based on the flowchart, what process does the box labeled "X" represent? a Fermentation b Cell disruption c Centrifugation 15 Based on the path of Product A vs. Product B, what can we infer about Product A? a It is an extracellular product. b It is an intracellular product. c It requires more processing than Product B.

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Downstream Processing: DP in Biotech

Froggy Jumps

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Créé par

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Downstream Processing: DP in BiotechVersion en ligne

par Nur Assyakirin binti Mohamed Paid

What is the primary function of solid-liquid separation?

Which technique is used to purify proteins based on binding properties?

Precipitation often recovers product by changing what?

What drying technique is common for heat-sensitive materials?

Why are downstream purification steps essential?

What is the stage that involves the cultivation and growth of microorganisms to produce desired products?

Which unit operations are commonly employed for solid-liquid separation in fermentation processes?

What is the purpose of disruption of cells in downstream processing?

What is the primary principle behind bead milling for cell disruption?

Which of the following is a method used for disruption of cells in downstream processing?

What is the common mechanism employed in homogenization to disrupt cells?

What is the primary mechanism of separation in Gel Filtration (size exclusion) chromatography?

How does Ion-exchange chromatography separate molecules within a sample?

Based on the flowchart, what process does the box labeled "X" represent?

Based on the path of Product A vs. Product B, what can we infer about Product A?