Expansion Microscopy - Difficulties quiz

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True/False statements about expansion microscopy.

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Diego

Spain

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Expansion Microscopy - Difficulties quizVersion en ligne True/False statements about expansion microscopy. par Diego 1 You need to carefully select the sample, as there is a compromise between long sample distances in z and small working distances in the microscope. Sí No 2 Gels should be as thick as possible. Sí No 3 A denaturing step is commonly used to homogenize the specimen for uniform expansion. Sí No 4 Gels must be handled with care to avoid geometrical defects and fractures. Sí No 5 It achieves higher lateral resolution by expanding the sample in space according to the swellable properties of the gel. Sí No 6 Due to the chemical anchoring process, no 100% sample might attach to the polymer network. Sí No 7 You can only visualize the expanded gel sample in an epifluorescence microscope. Sí No 8 It uses a swellable polymer hydrogel to physically expand the specimen. Sí No 9 Usually, it is easily bleached when using common fluorophores. Sí No 10 You can only do pre-staining. Sí No 11 Denaturing step can be only done with protease, such as proteinase K. Sí No 12 It isotropically expands samples to improve effective resolution. Sí No

Expansion Microscopy - Difficulties quizVersion en ligne

True/False statements about expansion microscopy.

par Diego

You need to carefully select the sample, as there is a compromise between long sample distances in z and small working distances in the microscope.

Sí

Gels should be as thick as possible.

Sí

A denaturing step is commonly used to homogenize the specimen for uniform expansion.

Sí

Gels must be handled with care to avoid geometrical defects and fractures.

Sí

It achieves higher lateral resolution by expanding the sample in space according to the swellable properties of the gel.

Sí

Due to the chemical anchoring process, no 100% sample might attach to the polymer network.

Sí

You can only visualize the expanded gel sample in an epifluorescence microscope.

Sí

It uses a swellable polymer hydrogel to physically expand the specimen.

Sí

Usually, it is easily bleached when using common fluorophores.

Sí

You can only do pre-staining.

Sí

Denaturing step can be only done with protease, such as proteinase K.

Sí

It isotropically expands samples to improve effective resolution.

Sí

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Expansion Microscopy - Difficulties quiz

Oui ou Non

À propos de cette activité

Créé par

Téléchargez la version pour jouer sur papier

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Expansion Microscopy - Difficulties quizVersion en ligne

par Diego

You need to carefully select the sample, as there is a compromise between long sample distances in z and small working distances in the microscope.

Gels should be as thick as possible.

A denaturing step is commonly used to homogenize the specimen for uniform expansion.

Gels must be handled with care to avoid geometrical defects and fractures.

It achieves higher lateral resolution by expanding the sample in space according to the swellable properties of the gel.

Due to the chemical anchoring process, no 100% sample might attach to the polymer network.

You can only visualize the expanded gel sample in an epifluorescence microscope.

It uses a swellable polymer hydrogel to physically expand the specimen.

Usually, it is easily bleached when using common fluorophores.

You can only do pre-staining.

Denaturing step can be only done with protease, such as proteinase K.

It isotropically expands samples to improve effective resolution.

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