Transcription & Translation Quiz 2

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Quiz on transcription and translation basics.

Créé par

Whitney Dunlap

United States

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Transcription & Translation Quiz 2


Transcription & Translation Quiz 2Version en ligne Quiz on transcription and translation basics. par Whitney Dunlap 1 Which enzyme is primarily responsible for synthesizing RNA during transcription in eukaryotic cells? a RNA polymerase 2 b RNA polymerase 1 c DNA polymerase 2 What is the function of the promoter region in transcription? a Guides ribosome assembly b Signals to RNA polymerase where to start c Signals to RNA polymerase where to end 3 Which strand of the double helix serves as the “template strand”? a Lagging strand b Leading strand c Complementary strand 4 What is a primary transcript? a The initial product of RNA transcription (pre-RNA) b A DNA template fragment c The final mature mRNA 5 What is the difference between an exon and an intron? a Exons stay in the nucleus, the introns exit the nucleus b Exons are non-coding sequences, introns code for proteins c Introns stay in the nucleus, the exons exit the nucleus 6 What catalyzes the formation of exons and introns? a DNA ligase b RNA polymerase c snRNP’s (snrps = “snips”) 7 What is topoisomerase’s job? a To relax DNA strand b To synthesize RNA c To unwind RNA 8 What is the role of ssbps? (single stranded binding proteins?) a To initiate transcription b To stabilize and protect DNA once it is split to prevent degradation and secondary structure formation c To cut DNA strands 9 Which direction is RNA synthesized? a 5’ 3’ b 3’ 5’ c 4’ 5’ 10 What is the TATA box? a Region of DNA that’s only A and T base pairs that act as the promoter region b A ribosomal RNA catalyst c A protein complex that initiates transcription 11 What must be added to pre-RNA before translation? a Polymerase II b 5’ cap and a poly A tail c A start codon and Shine-Dalgarno sequence 12 Where does translation take place? a Ribosome in cytosol b The nucleus c Mitochondria 13 Which site holds the growing peptide chain? a The A site b The E site c The P site 14 What are the different sites meant for in translation? a A: binding site, tRNA attaches; B: catalytic site, peptide bonds form; C: release site, finished polypeptide exits b A: acceptor site, tRNA carrying the right anticodon for mRNA codon enters here; b) P: peptidyl site, peptide bonds are formed between amino acids; c) E: tRNA’s without amino acids exit the ribosome c A: exit site, tRNA exits here; B: initiation site, where translation starts; C: compression site, tRNA is stabilized 15 True or false: translation does not require ATP a True b False c Only during initiation 16 What is one form of post-translational modification? a Folding, activation, degradation b RNA splicing c DNA replication 17 Where does transcription take place? a Mitochondria b A ribosome in the cytosol c Nucleus 18 What are the 3 stages of translation? a Initiation, capping, polyadenylation b Binding, translocation, termination c Initiation, elongation, termination 19 What is the role of tRNA? a To unwind DNA b To synthesize mRNA c To transfer amino acids to the ribosome to be matched to a specific codon 20 Why is it important to have an anticodon at the end loop of tRNA? a To catalyze peptide bond formation b This is what matches the base pairs of the mRNA codon c To bind to rRNA 21 What enzyme is in charge of “charging” tRNA by catalyzing the addition of the amino acids to tRNA’s? a DNA ligase b RNA polymerase II c Aminoacyl – tRNA synthases 22 What is a point mutation? a A duplication of an amino acid sequence b Where one specific nucleotide is altered c A deletion of a codon 23 What are some types of point mutations? a Insertion/deletion which lead to missense, non-sense, frameshift, silent b Chromosomal translocation c Gene duplication

Transcription & Translation Quiz 2

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Transcription & Translation Quiz 2Version en ligne

par Whitney Dunlap

Which enzyme is primarily responsible for synthesizing RNA during transcription in eukaryotic cells?

What is the function of the promoter region in transcription?

Which strand of the double helix serves as the “template strand”?

What is a primary transcript?

What is the difference between an exon and an intron?

What catalyzes the formation of exons and introns?

What is topoisomerase’s job?

What is the role of ssbps? (single stranded binding proteins?)

Which direction is RNA synthesized?

What is the TATA box?

What must be added to pre-RNA before translation?

Where does translation take place?

Which site holds the growing peptide chain?

What are the different sites meant for in translation?

True or false: translation does not require ATP

What is one form of post-translational modification?

Where does transcription take place?

What are the 3 stages of translation?

What is the role of tRNA?

Why is it important to have an anticodon at the end loop of tRNA?

What enzyme is in charge of “charging” tRNA by catalyzing the addition of the amino acids to tRNA’s?

What is a point mutation?

What are some types of point mutations?