eng genètica 3r

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biotec

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Mariona Miquel Garcia

Spain

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Grammys for best song/record

Educaplay Educational Resources

Spain

(50)

Answer if these songs won the Grammy for Best Song or Record of the Year (the general ones, category Grammys do not count)
Is it created with Artificial Intelligence? - Complete game

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Spain

(17)

Images, songs... Nothing is what it seems anymore. Are they generated with AI, or are they real?
Is it true?

Educaplay Educational Resources

Spain

(59)

Answer if the following sentences about general culture are true or not.
Adjective Checker

Erika Moreno

Ecuador

(14)

In this game, you will have to determine if the adjective provided accurately describes the noun. Test your knowledge of adjectives and see if you can spot the correct matches!
Foot and Ankle Anatomy Quiz

Thilaxshi Suresh

United Kingdom

(9)

Test your knowledge of the bones, joints, and muscles of the foot and ankle with this interactive quiz. Answer with ✅ if the statement is correct and ❌ if it is incorrect.

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eng genètica 3r


eng genètica 3rVersion en ligne biotec par Mariona Miquel Garcia 1 When designing a primer to introduce a change of amino acid in a protein, the mutation or mutations is/are in the center of the mutagenic primer Sí No 2 Klenow fragment can generate a new strand of DNA from an RNA template Sí No 3 In a PCR is always necessary to use degenerate primers Sí No 4 The hybridization probe for primer extension is a synthesized radio labeled oligonucleotide Sí No 5 In Real Time PCR we always use ûuorescent reporters to follow ampliûcation process Sí No 6 When producing a new recombinant protein, independently of your goals, you must always add an aûnity tag Sí No 7 Different restriction enzymes can generate the same sticky ends Sí No 8 Genomic libraries are constructed using cDNA Sí No 9 When working with lambda, replacement vectors accept less DNA than the insertion vectors Sí No 10 Polymerases requires a primer to synthese new DNA Sí No 11 Inducible promoters allow the regulation of the expression of particular cDNAs placed under their control Sí No 12 It is generally accepted that the optimal length of PCR product is between 18-22 bp Sí No 13 mRNA is easily puriûed from total RNA using oligodC aûnity columns Sí No 14 RNA seq uses next-generation sequencing to quantify DNA in a sample at a precise moment in time Sí No 15 An expression cassette consists on a promoter the coding sequences for a particular protein and a transcription termination signal Sí No 16 Linkers are attached to the end of sticky DNA molecule and can be cut to generate blunt ends Sí No 17 Cosmids are plasmids with an special module that permits the packaging of recombinant genomes into lambda particles Sí No 18 .The Gateway cloning technology is based on site-speciûc recombination system Sí No 19 The product of lambda particles containing a cosmic genome requires the lysis of infected cells that contain such genome Sí No 20 The yeast two-hybrid system is very useful but it does not allow to identify protein-protein interactions Sí No 21 At 94oC, DNA denaturates and strands are separated Sí No 22 In RNase protection assay you can only detect one RNA in each reaction Sí No 23 Phages derived from f1 that display a fusion protein in their surface cannot be generated so that they contain a genome with the DNA sequence that codes for that particular fusion protein Sí No 24 The signal peptide that is often included when producing recombinant proteins in Piccia pastors has the role of targeting the recombinant protein to the micocondria where it can accumulate withot tox Sí No 25 To identify recombinant colonies, one strategy is to replica plated onto agar medium that contains a second antibiotic, for instance, tetracycline Sí No 26 The gen called lacZ, encodes for beta-galactosidase, the enzyme involved in the breakdown of X-gal that give a deep blue colored product Sí No 27 Endonucleases removes nucleotides from the end of a DNA molecule Sí No 28 Polynucleotide kinase dephosphorilates 39-OH ends of DNA Sí No 29 RT-PCR allows you to compare gene expression between samples Sí No 30 Cell extracts used for in vitro translation provide ribosomes and tRNA Sí No 31 Sticky-end ligation is more eûcient than blunt-end ligation Sí No 32 In RACE PCR you can amplify a full length sequence of an RNA transcript Sí No 33 In real time PCR a house keeping gene is used as an internal control Sí No 34 Random priming is a good method to get speciûc labelling of one of the two DNA strands Sí No 35 To sequence the entire genome of an organism the most appropriate method is the Sanger method Sí No 36 All restriction endonucleases recognize hexanucleotide target sites and has a palindromic sequence Sí No 37 The northern blot is a technique used to study ene expression by detection of DNA in a sample Sí No 38 The Sanger method relies on the Watson and Crick base pairing Sí No 39 There exist several technological platforms for high-throughput sequencing. Depending on your needs one platform may be more appropriate than the other Sí No 40 PCR can be used for analyzing genotype or mouse models Sí No 41 Asymmetric PCR uses excess of only one primer and preferentially ampliûes one strand of the target DNA Sí No 42 Inducible promoters allow the regulation of the expression of particular cDNAs placed under their control Sí No 43 The substrate for DNA packaging in lambda particles ic circular DNA Sí No 44 Housekeeping gene has diûerent expression in normal or pathological conditions Sí No 45 Microarrays can be used to answer this question: Sí No 46 Haripins in primer loop are important for a correct binding to target DNA sequence Sí No 47 HIgher number of genes are detected in RNA seq versus Microarrays Sí No 48 The quick-change mutagenesis kit relies in methylation of the sequence GATC in E.coli and the lack of such modiûcation in newly in vitro-synthesized DNA when using PCR Sí No 49 In the Northern blot RNA is transferred from the electrophoresis agarose gel to the blotting nitrocellulose membrane Sí No 50 Multiplex-PCR uses one pair of primers annealing to diûerent target sequences Sí No 51 Depending on your goals or needs, cDNA libraries are prepared in diûerent vectors Sí No 52 In situ hybridization allows you to locate a fragment of nucleic acid whitin a histologic section Sí No 53 Choosing the appropriate homology arms allows precise integration of DNA sequences in the yeast genome Sí No 54 Oligo(dT) primers are used for cDNA synthesis reactions because of their speciûcity for mRNA Sí No 55 Lambda can undertake the lysogenic cycle only when the genome is recombinant Sí No 56 Northern blot could be used to detect expression of a speciûc gene in diûerent tissues Sí No

eng genètica 3r

Oui ou Non

À propos de cette activité

Créé par

Téléchargez la version pour jouer sur papier

Top Jeux

Oui ou Non

Grammys for best song/record

Oui ou Non

Is it created with Artificial Intelligence? - Complete game

Oui ou Non

Is it true?

Oui ou Non

Adjective Checker

Oui ou Non

Foot and Ankle Anatomy Quiz

eng genètica 3rVersion en ligne

par Mariona Miquel Garcia

When designing a primer to introduce a change of amino acid in a protein, the mutation or mutations is/are in the center of the mutagenic primer

Klenow fragment can generate a new strand of DNA from an RNA template

In a PCR is always necessary to use degenerate primers

The hybridization probe for primer extension is a synthesized radio labeled oligonucleotide

In Real Time PCR we always use ûuorescent reporters to follow ampliûcation process

When producing a new recombinant protein, independently of your goals, you must always add an aûnity tag

Different restriction enzymes can generate the same sticky ends

Genomic libraries are constructed using cDNA

When working with lambda, replacement vectors accept less DNA than the insertion vectors

Polymerases requires a primer to synthese new DNA

Inducible promoters allow the regulation of the expression of particular cDNAs placed under their control

It is generally accepted that the optimal length of PCR product is between 18-22 bp

mRNA is easily puriûed from total RNA using oligodC aûnity columns

RNA seq uses next-generation sequencing to quantify DNA in a sample at a precise moment in time

An expression cassette consists on a promoter the coding sequences for a particular protein and a transcription termination signal

Linkers are attached to the end of sticky DNA molecule and can be cut to generate blunt ends

Cosmids are plasmids with an special module that permits the packaging of recombinant genomes into lambda particles

.The Gateway cloning technology is based on site-speciûc recombination system

The product of lambda particles containing a cosmic genome requires the lysis of infected cells that contain such genome

The yeast two-hybrid system is very useful but it does not allow to identify protein-protein interactions

At 94oC, DNA denaturates and strands are separated

In RNase protection assay you can only detect one RNA in each reaction

Phages derived from f1 that display a fusion protein in their surface cannot be generated so that they contain a genome with the DNA sequence that codes for that particular fusion protein

The signal peptide that is often included when producing recombinant proteins in Piccia pastors has the role of targeting the recombinant protein to the micocondria where it can accumulate withot tox

To identify recombinant colonies, one strategy is to replica plated onto agar medium that contains a second antibiotic, for instance, tetracycline

The gen called lacZ, encodes for beta-galactosidase, the enzyme involved in the breakdown of X-gal that give a deep blue colored product

Endonucleases removes nucleotides from the end of a DNA molecule

Polynucleotide kinase dephosphorilates 39-OH ends of DNA

RT-PCR allows you to compare gene expression between samples

Cell extracts used for in vitro translation provide ribosomes and tRNA

Sticky-end ligation is more eûcient than blunt-end ligation

In RACE PCR you can amplify a full length sequence of an RNA transcript

In real time PCR a house keeping gene is used as an internal control

Random priming is a good method to get speciûc labelling of one of the two DNA strands

To sequence the entire genome of an organism the most appropriate method is the Sanger method

All restriction endonucleases recognize hexanucleotide target sites and has a palindromic sequence

The northern blot is a technique used to study ene expression by detection of DNA in a sample

The Sanger method relies on the Watson and Crick base pairing

There exist several technological platforms for high-throughput sequencing. Depending on your needs one platform may be more appropriate than the other

PCR can be used for analyzing genotype or mouse models

Asymmetric PCR uses excess of only one primer and preferentially ampliûes one strand of the target DNA

Inducible promoters allow the regulation of the expression of particular cDNAs placed under their control

The substrate for DNA packaging in lambda particles ic circular DNA

Housekeeping gene has diûerent expression in normal or pathological conditions

Microarrays can be used to answer this question:

Haripins in primer loop are important for a correct binding to target DNA sequence

HIgher number of genes are detected in RNA seq versus Microarrays

The quick-change mutagenesis kit relies in methylation of the sequence GATC in E.coli and the lack of such modiûcation in newly in vitro-synthesized DNA when using PCR

In the Northern blot RNA is transferred from the electrophoresis agarose gel to the blotting nitrocellulose membrane

Multiplex-PCR uses one pair of primers annealing to diûerent target sequences

Depending on your goals or needs, cDNA libraries are prepared in diûerent vectors

In situ hybridization allows you to locate a fragment of nucleic acid whitin a histologic section

Choosing the appropriate homology arms allows precise integration of DNA sequences in the yeast genome

Oligo(dT) primers are used for cDNA synthesis reactions because of their speciûcity for mRNA

Lambda can undertake the lysogenic cycle only when the genome is recombinant

Northern blot could be used to detect expression of a speciûc gene in diûerent tissues