Blue-White Screening Quiz

Froggy Jumps

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Test your gene cloning screening knowledge.

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Siti Nadira Nordin

United States

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Froggy Jumps Blue-White Screening QuizVersion en ligne Test your gene cloning screening knowledge. par Siti Nadira Nordin 1 In blue-white screening, what indicates a recombinant plasmid? a Colourless b White colonies c Blue colonies 2 Which enzyme activity is disrupted to form white colonies? a Restriction enzyme b Ligase c β-galactosidase 3 What substrate yields a blue color in non-recombinant colonies? a X-gal b plasmid c bacteria 4 In blue-white screening, the insert disrupts which gene? a lacZ b lacY c lacA 5 A blue colony indicates which type of plasmid? a Mutant plasmid b Non-recombinant c Recombinant with insert 6 What is the purpose of screening plates with an antibiotic? a Select killed bacteria b Selects transformed bacteria c Select non-transformed bacteria 7 Which condition favors X-gal hydrolysis to blue? a Intact lacZ b No lactose present c Disrupted lacZ 8 What happens to the bacteria that did not receive any foreign plasmid during the transformation of gene cloning? a do not form any colony on X-gal b form white colony in X-gal c form blue colony in X-gal 9 In the screening process, clones that metabolize X-gal turn into a blue b white c colorless 10 Which of the following genes in the following plasmid has a restriction site to cut open the plasmid? a AmpR b lacZ c Origin of replication

Froggy Jumps

Blue-White Screening QuizVersion en ligne

Test your gene cloning screening knowledge.

par Siti Nadira Nordin

In blue-white screening, what indicates a recombinant plasmid?

Colourless

White colonies

Blue colonies

Which enzyme activity is disrupted to form white colonies?

Restriction enzyme

Ligase

β-galactosidase

What substrate yields a blue color in non-recombinant colonies?

X-gal

plasmid

bacteria

In blue-white screening, the insert disrupts which gene?

lacZ

lacY

lacA

A blue colony indicates which type of plasmid?

Mutant plasmid

Non-recombinant

Recombinant with insert

What is the purpose of screening plates with an antibiotic?

Select killed bacteria

Selects transformed bacteria

Select non-transformed bacteria

Which condition favors X-gal hydrolysis to blue?

Intact lacZ

No lactose present

Disrupted lacZ

What happens to the bacteria that did not receive any foreign plasmid during the transformation of gene cloning?

do not form any colony on X-gal

form white colony in X-gal

form blue colony in X-gal

In the screening process, clones that metabolize X-gal turn into

blue

white

colorless

Which of the following genes in the following plasmid has a restriction site to cut open the plasmid?

AmpR

lacZ

Origin of replication

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Blue-White Screening Quiz

Froggy Jumps

À propos de cette activité

Créé par

Téléchargez la version pour jouer sur papier

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Blue-White Screening QuizVersion en ligne

par Siti Nadira Nordin

In blue-white screening, what indicates a recombinant plasmid?

Which enzyme activity is disrupted to form white colonies?

What substrate yields a blue color in non-recombinant colonies?

In blue-white screening, the insert disrupts which gene?

A blue colony indicates which type of plasmid?

What is the purpose of screening plates with an antibiotic?

Which condition favors X-gal hydrolysis to blue?

What happens to the bacteria that did not receive any foreign plasmid during the transformation of gene cloning?

In the screening process, clones that metabolize X-gal turn into

Which of the following genes in the following plasmid has a restriction site to cut open the plasmid?

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