Plant Improvement Quiz

Froggy Jumps

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Quiz on plant improvement.

Créé par

Carolina Arias

Mexico

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Plant Improvement Quiz


Froggy Jumps Plant Improvement QuizVersion en ligne Quiz on plant improvement. par Carolina Arias 1 Which approach do you choose when the trait already exists in relatives and you want to combine many genes without complex lab work? a Transgenesis b Cisgenesis c Selective crossing + MAS 2 If the trait does not exist in the crossable gene pool (e.g., Bt toxin), you use: a Induced mutagenesis b Cisgenesis c Transgenesis 3 If the trait exists in relatives and you want only that gene without dragging others, choose: a Cisgenesis b Transgenesis c Crossing + MAS 4 To create new variants without adding foreign DNA (then screen with TILLING), use: a RNAi b Induced mutagenesis c Transgenesis 5 To switch off/change a specific gene with precision and speed, use: a CRISPR (genome editing) b Crossing + MAS c RNAi 6 To lower a gene’s expression without changing its DNA sequence, use: a RNAi b Induced mutagenesis c Transgenesis 7 As a transformation method, first choice in many dicots with mature protocols: a Protoplasts b Agrobacterium c Biolistics 8 Method useful in cereals/difficult tissues and also for chloroplast: a Biolistics b Agrobacterium c Protoplasts 9 If your species regenerates well from protoplasts and you want to deliver CRISPR as RNPs (possible transgene-free editing), use: a Biolistics b Protoplasts with PEG/electroporation c Agrobacterium 10 TILLING is mainly used to: a Detect specific mutations in mutagenized lines b Insert bacterial genes c Silence a gene without touching its DNA 11 Goal “less browning” in fruit/mushroom: most direct strategy is: a Bt transgenesis b Cisgenes is of an R gene c RNAi or CRISPR targeting PPO 12 "Insect resistance" in a classic, proven way: a Editing the PPO promoter b Transgenesis with Bt c Cisgenesis of R genes 13 "Nutritional fortification" (e.g., β-carotene in endosperm) has typically been achieved with: a Protoplasts b Transgenesis c RNAi 14 "Fungal resistance": option consistent with the menu provided: a Bt transgenesis b Cisgenesis (R genes) or CRISPR knocking out susceptibility genes c RNAi against PPO 15 "Specific herbicide tolerance": classic approach mentioned: a Bt transgenesis b Induced mutagenesis (target-enzyme variants) c RNAi 16 Correct statement about Agrobacterium vs. biolistics: a Biolistics tends to insert multiple copies and can cause rearrangements b Both always integrate a single clean copy c Agrobacterium inserts more copies and more rearrangements 17 Key limitation of the protoplast route: a Does not allow electroporation b Regenerating whole plants can be difficult depending on genotype (totipotency) c Cannot deliver proteins 18 In CRISPR, “off-targets” refers to: a Fragments not integrating into the chloroplast b Undesired cuts/edits at sequences similar to the target c Genes expressed more than expected

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Plant Improvement Quiz

Froggy Jumps

À propos de cette activité

Créé par

Téléchargez la version pour jouer sur papier

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Plant Improvement QuizVersion en ligne

par Carolina Arias

Which approach do you choose when the trait already exists in relatives and you want to combine many genes without complex lab work?

If the trait does not exist in the crossable gene pool (e.g., Bt toxin), you use:

If the trait exists in relatives and you want only that gene without dragging others, choose:

To create new variants without adding foreign DNA (then screen with TILLING), use:

To switch off/change a specific gene with precision and speed, use:

To lower a gene’s expression without changing its DNA sequence, use:

As a transformation method, first choice in many dicots with mature protocols:

Method useful in cereals/difficult tissues and also for chloroplast:

If your species regenerates well from protoplasts and you want to deliver CRISPR as RNPs (possible transgene-free editing), use:

TILLING is mainly used to:

Goal “less browning” in fruit/mushroom: most direct strategy is:

"Insect resistance" in a classic, proven way:

"Nutritional fortification" (e.g., β-carotene in endosperm) has typically been achieved with:

"Fungal resistance": option consistent with the menu provided:

"Specific herbicide tolerance": classic approach mentioned:

Correct statement about Agrobacterium vs. biolistics:

Key limitation of the protoplast route:

In CRISPR, “off-targets” refers to: