Relier Pairs Which microscopic Image fits?Version en ligne A fun way to remember the correct microscopy for viewing tissues. par Taylor Shelton 1 Definition of Transmission Electron Microscopy 2 Definition of Electron Microscopy 3 Definition of Conventional Light Microscopy 4 Image of conventional light BRIGHT FIELD microscopy 5 IMAGE of bright-field microscopy, collagen fibers that appear red, with thin elastic fibers with cell nuclei. 6 Definition of Polarizing Microscopy 7 Definition of Autoradiography 8 Definition of Confocal Microscopy 9 Definition of Phase-Contrast Microscopy 10 Definition of a Scanning Electron Microscopy 11 Definition of Fluorescence Microscopy 12 IMAGE of phase-contrast microscopy 13 IMAGE of polarizing microscopy only showing collagen fibers with intense yellow or orange birefringence. 14 IMAGE of TEM of a kidney cell with acid phosphatase. 15 Periodic acid-schiff (PAS) 16 IMAGE of confocal microscope 17 IMAGE of Transmission Electron Microscopy & Autoradiography 18 IMAGE of fluorescence, uterine cell stain 19 IMAGE of contrast microscopy Can be used with confocal light and irradiation of tissues using UV light or a laser Regular light microscope/ bright field Reaction stains polysaccharides CAN see 3D images. Electron beam does not pass through the specimen but is scanned across its surface. Gives a greater resolution and is widely used in research used to dissect tissues optically and can also use fluorescence Can NOT see 3D images. uses the interaction of a beam of electrons with a specimen to produce a image Used for physics and can rotate the angle of the plane of polarized light Can see parts of the cell and at a higher resolution Used to see binding receptors and used to localize radioactive material within the tissue 3D type for ex. looking at cells
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