Relier Pairs Tissue Prep Steps and Acid DyeVersion en ligne Creative way to know histological terms. par Taylor Shelton 1 Fixation 2 Clearing 3 Infiltration 4 Embedding 5 Trimming 6 Cationic components such as proteins stain what kind of dye? 7 Nucleic acids that have negative charges (anionic) use what kind of dye? 8 Dehydration The resulting paraffin block is trimmed to expose the tissue for sectioning (slicing) on a microtome The tissue is transferred through a series of increasingly concentrated alcohol solutions, ending in 100%, which REMOVES ALL WATER The tissue is placed in melted paraffin until it becomes completely infiltrated with this substance Small pieces of tissue that are placed in solutions of chemicals that cross-link proteins and inactive degradative enzymes, which PRESERVES CELL AND TISSUE STRUCTURE Acidophillic Basophillic Alcohol is removed in organic solvents in which both alcohol and paraffin are alike (miscible) The paraffin-infiltrated tissue is placed in a small mold with melted paraffin and allowed to harden
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