Relier Pairs Histology Chapter 1Version en ligne A fun and creative way to remember histology and its methods. par Taylor Shelton 1 Dehydration does what? 2 polarizing 3 What increases concentration of alcohol? 4 Sudan black stain 5 Peroxidase 6 phase contrast 7 cell structures attract what colored dye? 8 What does the fixation step do? 9 conventional light 10 rehydration is used to do what? 11 Calcium ions in calcified bone 12 Two types of tissue preparation 13 proteins attract what kind of dye? 14 Name 5 fixatives 15 Two Tissue Components 16 What decreases the concentration of alcohol? 17 Normarski 18 What does embedding do? 19 Alkaline Phosphatase 20 Sectioning 21 what does clearing do? 22 4 tissue types 23 staining 24 what stains sugars? Acid dye with positive charge. 10% Formalin 4% paraformaldehyde 70% Ethanol Methonal Glutaraldehyde and Osmium Tetroxide (EM) Infiltrates tissue with solid medium to facilitate sectioning PAS- Periodic Acid- Schiff reaction Epithelial Connective Muscular Nervous Cut tissue into thin slices of about 10 micrometers. used to visualize structural features Paraffin-embedded tissue Frozen tissue using alcohol to get out access fluid In xylene to embed in tissue Remove paraffin Cells Extracellular Matrix rehydration Dehydration It preserves tissue structure basic dye with negative charges
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