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Recall the difference between the intracellular and intercellular environment.
Differentiate between macrotransfer and microtransfer processes.
Explain the mechanism of both phagocytosis and pinocytosis.
Differentiate between receptor-mediated and fluid phase pinocytosis.
Describe passive diffusion processes and the role of membrane transport proteins.
Differentiate between transporter and channel proteins.
Explain facilitated transport.
Describe mechanisms of active transport in cell membranes.
Explain how glucose enters the cell by means of a sodium dependent co-transport carrier system.
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FUNBIO 11 part 2
Recall the difference between the intracellular and intercellular environment.
Differentiate between macrotransfer and microtransfer processes.
Explain the mechanism of both phagocytosis and pinocytosis.
Differentiate between receptor-mediated and fluid phase pinocytosis.
Describe passive diffusion processes and the role of membrane transport proteins.
Differentiate between transporter and channel proteins.
Explain facilitated transport.
Describe mechanisms of active transport in cell membranes.
Explain how glucose enters the cell by means of a sodium dependent co-transport carrier system.
1
mediated
postulate
tendency
kinetic
Microtransfer
antiport
random
Na
CO2
ceases
3
permeable
macrophage
electrochemical
endosomes
Triphosphate
Brownian
Glucose
are ATPase proteins
facilitated
charged
A
large
can
engulf
/
endocytose
25%
of
its
own
volume
of
fluid
each
hour
by
the
process
of
pinocytosis
This
removes
3%
of
its
plasma
membrane
each
minute
(
100%
of
its
plasma
membrane
per
hour
)
The
total
surface
area
and
volume
of
the
cell
remains
unchanged
This
means
that
exocytosis
must
balance
endocytosis
with
respect
to
the
amount
of
membrane
involved
Macrotransfer
only
brings
materials
into
or
lysosomes
within
the
cytoplasm
For
molecules
to
be
absorbed
into
the
cytoplasm
they
must
cross
the
surrounding
membrane
is
the
transfer
of
small
molecules
and
ions
across
cell
membranes
,
both
internal
and
external
Microtransfer
of
biomolecules
can
be
by
two
different
mechanisms
:
-
Passive
transport
No
energy
required
Simple
and
diffusion
-
Active
transport
Requires
energy
Primary
and
secondary
active
transport
Many
ions
and
small
molecules
move
through
the
membrane
by
diffusion
.
Differentiate
between
Brownian
motion
and
diffusion
:
Brownian
motion
:
-
First
observed
indirectly
by
the
Scottish
botanist
Robert
Brown
.
-
Under
the
light
microscope
,
pollen
grains
in
water
are
seen
to
be
moving
here
and
there
(
movement
)
in
water
.
So
called
Motion
applies
to
particles
<2
micrometers
and
does
not
require
energy
consumption
-
It
is
due
to
collisions
with
the
water
molecules
which
we
can
?
t
see
.
Diffusion
The
for
all
molecules
in
liquids
and
gases
to
move
in
all
directions
until
they
are
evenly
distributed
in
the
available
space
-
is
dependent
on
movement
of
molecules
,
due
to
their
energy
,
from
regions
of
high
concentration
to
low
concentration
-
It
will
occur
whenever
such
a
concentration
gradient
exists
-
Movement
when
the
equilibrium
constant
reaches
0
There
are
two
types
of
diffusion
:
(
simple
)
diffusion
?
through
the
lipid
bilayer
Facilitated
diffusion
(
or
transport
)
?
through
a
channel
protein
or
carrier
protein
simple
diffusion
:
-
This
requires
the
membrane
to
be
freely
to
the
molecules
and
ions
at
the
time
in
question
-
Water
and
small
non
-
polar
molecules
(
O2
,
)
pass
freely
through
the
plasma
membrane
by
diffusion
(
Molecules
diffuse
across
the
membrane
by
passive
transport
)
facilitated
diffusion
/
transport
:
and
other
molecules
(
e
.
g
.
amino
acids
,
nucleotides
)
enter
some
animal
cells
by
a
process
of
facilitated
(
or
)
diffusion
Two
types
of
protein
facilitate
the
diffusion
,
and
each
one
uses
a
different
mechanisms
:
Channel
proteins
(
e
.
g
.
potassium
ion
channels
)
Carrier
proteins
(
e
.
g
.
sugars
,
amino
acids
and
nucleotides
)
are
specific
permeases
which
are
highly
selective
often
transporting
only
one
type
of
molecule
.
The
mechanism
involves
a
conformational
changes
to
transfer
the
bound
solute
No
energy
is
required
for
transport
;
essentially
this
is
a
passive
transport
mechanism
Macromolecules
and
ions
(
H
+
,
Na
+
,
K
+
,
Cl
-
)
do
not
pass
freely
through
cell
membranes
.
Instead
these
molecules
require
the
use
of
selective
pores
or
channels
(
so
called
?
membrane
transport
proteins
?
)
to
cross
/
transfer
from
one
side
of
the
membrane
to
the
other
-
Most
models
that
integral
transmembrane
proteins
act
as
carriers
translocating
substances
from
one
surface
to
another
-
These
active
transport
systems
are
usually
inhibited
by
low
temperature
The
process
is
energy
dependent
and
requires
Adenosine
(
ATP
)
molecules
The
best
studied
active
transport
systems
involve
inorganic
ions
,
amino
acids
and
monosaccharides
Two
types
of
active
transport
both
of
which
require
energy
expenditure
in
order
to
transport
molecules
AGAINST
their
concentration
gradient
.
Primary
?
uses
energy
(
ATP
)
directly
Secondary
?
uses
energy
indirectly
.
Typically
move
molecules
against
their
electrochemical
gradient
.
Uses
energy
in
the
form
of
ATP
Primary
active
transporters
are ATPase proteins
.
Primary
active
transporters
are
also
referred
to
as pumps
.
primary
active
transport
:
Typically
move
molecules
against
their
electrochemical
gradient
.
Uses
energy
in
the
form
of
ATP
-
Primary
active
transporters
.
-
Primary
active
transporters
are
also
referred
to
as pumps
.
Examples
of
substances
transported
by
this
mechanism
:
Na
+
,
K
+
,
Ca
+
+
and
H
+
Examples
of
pumps
:
Na
+
/
K
+
-
ATPase
,
Ca2
+
-
ATPase
,
H
+
/
K
+
-
ATPase
,
H
+
-
ATPase
pumps
,
ATP
synthase
(
in
reverse
)
note
that
the
gradient
established
by
primary
active
transport
stores
energy
that
can
be
used
to
drive
other
transport
systems
?
we
will
see
this
as
a
key
mechanism
in
Secondary
Active
transport
Primary
Active
transport
:
Sodium
potassium
pump
Na
+
/
K
+
-
ATPase
Most
membranes
incorporate
an
ATP
-
driven
sodium
/
potassium
pump
(
Na
+
/
K
+
-
ATPase
)
,
which
maintains
a
+
gradient
.
This
pump
transports
:
Na
+
ions
from
inside
to
out
2
K
+
from
outside
to
in
1
ATP
hydrolysed
(
may
be
considered
an
example
of
)
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