Clearing
Trimming
Infiltration
Dehydration
Nucleic acids that have negative charges (anionic) use what kind of dye?
Cationic components such as proteins stain what kind of dye?
Embedding
Fixation
The paraffin-infiltrated tissue is placed in a small mold with melted paraffin and allowed to harden
Acidophillic
The tissue is placed in melted paraffin until it becomes completely infiltrated with this substance
The tissue is transferred through a series of increasingly concentrated alcohol solutions, ending in 100%, which REMOVES ALL WATER
The resulting paraffin block is trimmed to expose the tissue for sectioning (slicing) on a microtome
Small pieces of tissue that are placed in solutions of chemicals that cross-link proteins and inactive degradative enzymes, which PRESERVES CELL AND TISSUE STRUCTURE
Basophillic
Alcohol is removed in organic solvents in which both alcohol and paraffin are alike (miscible)
