recognition bacteriophage cloning EcoRI restrict overhangs replicate marker anneal origin staggered foreign ampR
i
)
Vectors
are
agents
of
carrying
foreign
DNA
segment
.
They
are
able
to
freely
/
autonomously
because
they
have
their
own
of
replication
.
They
possess
multiple
sites
(
MCS
)
to
receive
DNA
.
They
are
able
to
express
these
foreign
DNA
.
They
possess
genes
for
screening
such
as
ampicillin
resistance
(
)
.
Some
of
cloning
vector
examples
are
plasmid
,
and
cosmid
.
ii
)
Palindrome
is
when
the
same
base
sequence
is
read
in
opposite
direction
on
a
double
stranded
DNA
.
Palindrome
forms
sites
for
restriction
enzymes
,
which
will
both
of
the
DNA
strands
.
iii
)
Sticky
ends
are
produced
when
the
restriction
enzyme
produce
cut
.
This
will
produce
,
which
is
hanging
complementary
single
strand
of
DNA
at
the
cut
ends
.
DNA
with
complementary
sticky
ends
are
able
to
/
join
.
Some
examples
of
enzymes
that
produce
sticky
end
are
BamHI
and
.